This page was produced as an assignment for Genetics 564, an undergraduate capstone course at The University of Wisconsin - Madison.
What is chemical genetics?
Proteins can interact with small molecules. In chemical genetics, the function of proteins and signal transduction pathways in cells are analyzed by the screening of chemical libraries of small molecules . To quantify the interactions of proteins and small molecules, a chemical genetic screen is used to identify which small molecules interact with the protein of interest by screening it against a library of small molecules.
Developing a chemical genetic screen for APP
Since the Amyloid Precursor Protein (APP) plays a significant role in the Amyloid Cascade Hypothesis an in Alzheimer's disease, developing a chemical genetic screen against three Alzheimer's drugs could be used to identify potential inconsistencies in a mutant APP. The use of Methionine sulfoxide reductase A, Florbetaben, and Flutemetamol are proposed to compare the solubility of mutant promoter protein products with the wildtype.
This proposed chemical genetic screen could indicate that the biochemical properties of the mutant promoter protein products produced in Aim 1 are biochemically consistent. This information could be used to further characterize the upstream promoter region of APP.
 Jaroch, S., & Weinmann, H. (Eds.). (2007). Chemical genomics: small molecule probes to study cellular function (Vol. 58). Springer Science & Business Media.